Abstract
The neuropathy target esterase is a membrane-bound enzyme linked to organophosphate-induced distal neuropathy. Here we report a tentative mechanism of its solubilization from chicken embryo brains by using phospholipase A2. The enzyme was released from brain membranes after degradation of their structural phospholipids initiated by phospholipase A2. L-α-lysophosphatidylcholine, tested as a representative product of phospholipid hydrolysis, was identified as a new efficient detergent for solubilization of the neuropathy target esterase.
Highlights
The neuropathy target esterase (NTE) is a neural, membrane-bound, phenyl valerate carboxylesterase (PVC) linked to etiology of organophosphate-induced distal neuropathy[1,2]
More than 90% of NTE activity has been recovered from chicken embryo brain membranes by using phospholipase A2 (PLA2)[5]
In the work reported here, we examined the consequences of PLA2 treatment of chicken embryo brain membranes on membrane phospholipids and a possible involvement of lysophospholipids in NTE solubilization
Summary
The neuropathy target esterase (NTE) is a neural, membrane-bound, phenyl valerate carboxylesterase (PVC) linked to etiology of organophosphate-induced distal neuropathy[1,2]. NTE can be solubilized from brain membrane preparations with ionic or nonionic detergents[3,4], but often with significant losses of enzyme activity. More than 90% of NTE activity has been recovered from chicken embryo brain membranes by using phospholipase A2 (PLA2)[5]. In the work reported here, we examined the consequences of PLA2 treatment of chicken embryo brain membranes on membrane phospholipids and a possible involvement of lysophospholipids in NTE solubilization.
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