Abstract
This work described a temperature-triggered fiber optic biosensor for sequential detection of cholesterol and glucose based on poly(N-isopropylacrylamide)-co-acrylamide)(P(NIPAAm-co-AAm))-magnetic immobilized glucose oxidase(GOD) complex (PMIGC) and magnetic immobilized cholesterol oxidase (COD). At 38°C, the sensor will selectively detect cholesterol concentration with the detection range of 25–250mg/dL since P(NIPAAm-co-AAm) is known to shrink above its lower critical solution temperature (LCST) of 36°C, and will separate GOD from analytes so that PMIGC has no catalysis effect on glucose. When the temperature was switched to 25°C (below LCST), PMIGC could catalyze the oxidation of glucose since GOD will be exposed to analytes due to the swell of P(NIPAAm-co-AAm). This sensor can be used for the detection of glucose with the detection range of 50–700mg/dL. The optimal detection conditions for cholesterol were achieved with pH 7.0, 40°C and 10mg COD (in 75mg carrier), and those for glucose were achieved with pH6.5, 35°C and 12mg GOD (in 90mg carrier). The biosensor proposed is shown to have outstanding repeatability, selectivity and yield satisfactory detection results on practical samples.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
