Abstract
A split-stem technique was devised for the rapid evaluation of herbicide effect on root growth and herbicide translocation. Four rows of adventitious root initials were prominent on the stem of red kidney bean [Phaseolus vulgaris L.] seedlings. They were excised at the soil level above the vascular plate. Excised stems were longitudinally split for a distance of 75 to 90 mm leaving two rows of root initials on each stem portion. Split-stem halves were immersed in herbicide-nutrient and nutrient solutions, respectively, contained in paired 25 by 150-mm test tubes. Subsequent plant growth and development of treated plants reflected effects of the herbicide on root growth. Herbicide translocation often was indicated by abnormalities of growth of plant tissues remote from the herbicide solution.
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