A Technique for Sequential Examination of in Vitro Macrophage-Tumor Interaction using Lm, Sem and Tem

Abstract

A sequential examination of macrophage-tumor cell interaction in vitro, using time-lapse cinematography followed by SEM and TEM of the same cells, is a valuable asset in the interpretation of in vivo and in vitro cell-mediated cytotoxicity assays that are widely used in tumor immunology. Previous reports on sequential examination of biological specimens involved large tissue blocks. This report describes a technique that allows sequential examination of cell - cell interaction using three modes of microscopy.Guinea pig hepatoma (L-10) cells were plated sparsely on 25mm glass coverslips 24hr before adding guinea pig peritoneal exudate cells (PEC). PEC were allowed to settle for 1 l/2hr; the coverslips were then transferred to a Dvorak-Stotler chamber and examined with a Zeiss photomicroscope III.