A technique for embryo culture of peaches

Abstract

This publication describes a technique. … for growing peach-embryo cultures. In laboratory experiments, a high percentage of the cultures was free of microorganisms that would develop under yeast-extract broth within 15 days at room temperature. The salient feature of the technique is a “sterilization” procedure on dry, dormant peach seeds, followed by maintenance of aseptic conditions during the subsequent after-ripening. Merthiolate 1:2,000 in 50 per cent alcohol was found to be suitable for the purpose. Various factors influencing the growth of peach-embryo cultures are discussed. Suggestions are also made for establishing a standard practice in growing peach-embryo cultures to be used for biological testing.