Abstract
The methods that consist in growing fungal mycelia on cellophane sheets, in order to get longitudinal sections for electron microscopy, show some insufficiencies. It is possible to use a derived procedure from Kuhner's method of culture under a collodion film. This can be done by growing the mycelium in a drop of nutrient broth, squashed between two collodion films. The upper film prevents the mycelium from emitting aerial hyphae, and obliges it to grow only in a horizontal thin layer. The lower film does not adhere to the support and allows an easy harvesting of the specimen. Fixation and dehydratation are made on the whole culture assembly. Then, little pieces cut from this assembly are infiltrated with resin, included and sectionned.
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