Abstract
Abstract A tandem Aldol condensation/dehydration of aldehydes and ketones could be performed under d -aminoacylase and N -heterocyclic compounds used as co-catalyst in organic media. Some control experiments have been designed to demonstrate that either acylase or N -heterocyclic compounds could not catalyze the tandem reaction. The acylase showed the highest activity in the presence of imidazole and has been used to catalyze the tandem Aldol condensation/dehydration between different aldehydes and ketones. This method has provided a new strategy to perform the tandem Aldol condensation/dehydration and expanded the application of biocatalysts.
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