Abstract
Runting and stunting syndrome (RSS), which is characterized by lower body weight, widely occurs in broilers. Some RSS chickens simply exhibit slow growth without pathological changes. An increasing number of studies indicate that broiler strains differ in susceptibility to infectious diseases, most likely due to their genetic differences. The objective of this study was to detect the differentially expressed miRNAs and mRNAs in RSS and normal chickens. By integrating miRNA with mRNA expression profiling, potential molecular mechanisms involved in RSS could be further explored. Twenty-two known miRNAs and 1,159 genes were differentially expressed in RSS chickens compared with normal chickens (P < 0.05). qPCR validation results displayed similar patterns. The differentially expressed genes were primarily involved in energy metabolism pathways. The antisense transcripts were extensively expressed in chicken liver albeit with reduced abundance. Dual-luciferase reporter assay indicated that gga-miR-30b/c directly target CARS through binding to its 3′UTR. The miR-30b/c: CARS regulation mainly occurred in liver. In thigh muscle and the hypothalamus, miR-30b/c are expressed at higher levels in RSS chickens compared with normal chickens from 2 to 6 w of age, and notably significant differences are observed at 4 w of age.
Highlights
Runting and stunting syndrome (RSS), which is characterized by low body weight, mainly occurs in broilers, and the condition is incredibly complicated [1,2,3]
An increasing number of studies indicate that broiler strains differ in susceptibility to infectious diseases, most likely due to their genetic differences [9,10,11]
Five negative RSS chicken and 5 normal healthy individuals were used for Solexa and Digital Gene Expression (DGE) sequencing
Summary
Runting and stunting syndrome (RSS), which is characterized by low body weight, mainly occurs in broilers, and the condition is incredibly complicated [1,2,3]. Previous reports indicate that both genetic and environmental factors, such as diseases and feeding, are implicated in arrested development [4, 5]. The body weights of RSS chickens are significantly reduced compared with normal chickens. RSS generally occurs early in life and always results in PLOS ONE | DOI:10.1371/journal.pone.0127342. MRNA and MicroRNA Profilings in Runting and Stunting Chickens Liver RSS generally occurs early in life and always results in PLOS ONE | DOI:10.1371/journal.pone.0127342 May 26, 2015
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