Abstract
A method for the analysis of hydrocarbons in exhaled human breath samples has been developed and its quantitative performance optimized and exhaustively validated. The method involves preconcentration on a solid absorbent at 0 degree C and desorption at 250 degrees C to a packed column gas chromatograph. Calibrations for ethane and pentane are reproducible and linear over the concentration ranges found in human breath samples. The technique is now available for study of conditions, such as cystic fibrosis, in which an oxidative stress component in tissue injury is suspected.
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