Abstract
BIOCHEMICAL studies of the Trypanosomidae have been delayed by the difficulties encountered in cultivation and maintenance of many species. Up to the present time, only one member of this family (Herpetomonas culicidarum) has been cultivated in a defined medium1, and this was of a degree of complexity comparable to that required by the most exacting bacteria. The present communication describes a simple synthetic medium which will support the growth of the parasitic flagellate Strigomonas oncopelti; this trypanosomid, first isolated in bacteria-free culture by Noguchi and Tilden2 in 1926, is parasitic in the digestive tract of Hemiptera and in latex plants. Lwoff3 reported that Strigomonas oncopelti could be cultivated in alkaline hydrolysates of silk or in certain peptones if these were supplemented with thiamine ; in contrast to other trypanosomids, it does not require haematin as a growth factor.
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