A Synergistic Sensitized Fluorescent Determination of 2,4-Dichlorophenoxyacetic Acid in Vegetable Samples Based on the Derivatives of Calix[4]arene

Xiashi Zhu,Qiuyi Ren

doi:10.22158/fsns.v3n4p142

Abstract

A novel fluorescent determination of 2,4-dichlorophenoxyacetic acid (2,4-D) based on the derivatives of calix[4]arene (SAX) in ?-cyclodextrin(?-CD)/ sodium dodecyl sulfate (SDS) synergistic sensitized system was developed. The results were shown that the fluorescence intensity of SAX could be quenched by 2,4-D, and the fluorescence quenching (?F=FSAX-F2,4-D -SAX) was synergistic sensitized in ?-CD/ SDS medium. Under the conditions of ?ex/em=332/468 nm and pH 7.0, the linear range for 2,4-D were found to be 0.020-4.00 ?g/mL. The mechanism of determination was discussed with quenching type analysis, inclusion interaction and sensitizing effect. This method has been applied for the determination of 2,4-D in vegetable samples with satisfactory results.

Highlights

A novel fluorescent determination of 2,4-dichlorophenoxyacetic acid (2,4-D) based on the derivatives of calix[4]arene (SAX) in β-cyclodextrin(β-CD)/ sodium dodecyl sulfate (SDS) synergistic sensitized system was developed
The results were shown that the fluorescence intensity of SAX could be quenched by 2,4-D, and the fluorescence quenching (∆F=FSAX-F2,4-D -SAX) was synergistic sensitized in β-CD/ SDS medium
The cavity of SAX is composed of four benzene rings, meeting the size and stability required for the inclusion interaction

Summary

Introduction

A novel fluorescent determination of 2,4-dichlorophenoxyacetic acid (2,4-D) based on the derivatives of calix[4]arene (SAX) in β-cyclodextrin(β-CD)/ sodium dodecyl sulfate (SDS) synergistic sensitized system was developed. The mechanism of determination was discussed with quenching type analysis, inclusion interaction and sensitizing effect This method has been applied for the determination of 2,4-D in vegetable samples with satisfactory results. Schiff base calix[4]arene (SAX, Figure 1(b)) has been followed with great interests due to the simple structure and high symmetry. The fluorescence quenching methods using schiff base calix[4]arene derivatives as a fluorescent chemosensor for the determination of 2,4-D seems to be lacking. 100.0 μg/mL stock solution of 2,4-dichlorophenoxyacetic acid (2,4-D) was prepared by dissolving 0.100g 2,4-D in 100 mL volumetric flask and diluting with anhydrous ethanol to scale. A UV 2501 spectrophotometer (Shimadzu, Japan) was used for all absorption spectral recordings and absorbance measurements

Methods

Results

Conclusion