A synbiotic of Anaerostipes caccae and lactulose prevents and treats food allergy in mice

Abstract

Depletion of beneficial microbes by modern lifestyle factors correlates with the rising prevalence of food allergies. Re-introduction of allergy-protective bacteria may be an effective treatment strategy. We characterized the fecal microbiota of healthy and food-allergic infants and found that the anaerobe Anaerostipes caccae (A.caccae) was representative of the protective capacity of the healthy microbiota. We isolated a strain of A.caccae from the feces of a healthy infant and identified lactulose as a prebiotic to optimize butyrate production by A.caccae invitro. Administration of a synbiotic composed of our isolated A.caccae strain and lactulose increased luminal butyrate in gnotobiotic mice colonized with feces from an allergic infant and in antibiotic-treated specific pathogen-free (SPF) mice, and prevented or treated an anaphylactic response to allergen challenge. The synbiotic's efficacy in two models and microbial contexts suggests that it may be a promising approach for the treatment of food allergy.