A switchable peptide sensor for real-time lysosomal tracking

Lei Chen,He Tian,Carsten Schmuck,Junchen Wu

doi:10.1039/c4cc00670d

Lei Chen, He Tian + Show 2 more

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https://doi.org/10.1039/c4cc00670d

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Abstract

A bis-spiropyran functionalized peptide 1, which exhibits good cell-permeability, excellent biocompatibility and low cytotoxicity, has been developed for reversible and real-time lysosomal tracking.

Highlights

A bis-spiropyran functionalized peptide 1, which exhibits good cellpermeability, excellent biocompatibility and low cytotoxicity, has been developed for reversible and real-time lysosomal tracking
It should be noted that the intrinsic photoswitchable property of spiropyran could be achieved by either pH or Vis-light realizing reversible lysosomal tracking
Peptide 1 was synthesized using a microwave-assisted solid-phase peptide method that is commonly used for peptide-based biosensors,[14] which was isolated by preparative HPLC on a reversed-phase C-18 column in 99% purity and 25% yield, respectively (ESI†)

Summary

Introduction

A bis-spiropyran functionalized peptide 1, which exhibits good cellpermeability, excellent biocompatibility and low cytotoxicity, has been developed for reversible and real-time lysosomal tracking. It should be noted that the intrinsic photoswitchable property of spiropyran could be achieved by either pH or Vis-light realizing reversible lysosomal tracking.

Results

Conclusion