Abstract
Twenty four ryegrass /white clover pastures throughout Otago and Southland were surveyed for the presence of ryegrass mosaic virus (RgMV),Acremonium lolii and eriophyid mites. Densities of Argentine stem weevil were measured at eight sites. RgMV was found to be widespread and was recorded from 15 locations for the first time. Infection of up to 48% of ryegrass tillers by RgMV was measured. A. lolii was also found to be widespread with levels of up to 94% recorded. Mites were found at six sites. No correlation of RgMV incidence with A. lolii, presence of mites or Argentine stem weevil densities was detected. A comparison of A. lolii detection by ELISA and epidermal staining was carried out. The ELISA technique appeared to have greater sensitivity.
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