Abstract
AbstractThe reaction to Tomato spotted wilt virus (TSWV) was evaluated in 94 accessions of Nicotiana, originating from the Institute of Soil Science and Plant Cultivation tobacco germplasm collection in Puławy, Poland. Tests for resistance were conducted under greenhouse conditions using single TSWV isolate collected from tobacco plantation in Lublin district, Poland. The presence of the virus was verified using DAS-ELISA. SCAR markers associated with TSWV resistance gene were applied. The members of the section Alatae, the genus Nicotiana: N. alata, N. forgetiana, and Nicotiana x sanderae as well as N. tabacum cultivars: ‘Polalta’ and ‘Wiktoria’ with the TSWV resistance gene introduced from N. alata, displayed the hypersensitive reaction (HR) against TSWV (grade 0 on symptom intensity scale). In some of those accessions, the virus spread from the initially infected areas eliciting systemic hypersensitive reaction (SHR). Five accessions of N. alata and three of Nicotianaxsanderae were composed of 6.3–50.0 % of plants in which SHR symptoms appeared. In all of N. forgetiana plants HR reaction was followed by systemic infection (SHR). In N. tabacum ‘Wiktoria’ 21.1 % of plants showed HR reaction, while the remaining were susceptible (S). All of the genotypes which responded with HR or SHR reaction to TSWV infection demonstrated the presence of SCAR markers linked to the resistance gene. The remaining eighty tested accessions were identified as being susceptible upon exposure to TSWV.
Highlights
The genus Nicotiana is one of the most numerous in the family Solanaceae
The members of the section Alatae, the genus Nicotiana: N. alata, N. forgetiana, and Nicotiana x sanderae as well as N. tabacum cultivars: ‘Polalta’ and ‘Wiktoria’ with the Tomato spotted wilt virus (TSWV) resistance gene introduced from N. alata, displayed the hypersensitive reaction (HR) against TSWV
In some plants of those accessions local lesions were subsequently accompanied by chlorotic spots, and in 14 days the systemic infection set in, at first manifested as fastgrowing and coalescing chlorotic necrotic spots, which led to leaf and apex distortion (Table 1)
Summary
Nicotiana species are native to North and South America, Australia, some Pacific islands and Namibia in Africa. Such an extensive area of origin and evolutionary heterogeneity makes Nicotiana an extremely diversified group. A revised systematics based on molecular research was proposed (Chase et al 2003; Clarkson et al 2004; Knapp et al 2004). In the new classification by Knapp et al (2004) subgenera were dropped and only the division into sections was retained. According to the new classification, N. trigonophylla Dun. was renamed N. obtusifolia Martens et Galeotti, N. affinis Hort is synonymous with N. alata Link et Otto, and N. bigelovii (Torrey) Watson with N. quadrivalvis Pursh. N. sanderae Hort. is currently regarded to be hybrid between N. alata and N. forgetiana Hemsl. (Nicotiana x sanderae) whereas N. eastii Kostoff is an autotetraploid variant of N. suaveolens Lehm. (Chase et al 2003; Knapp et al 2004)
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