Abstract
Adhesion to epithelial cells is considered important for Lactobacillus to exert probiotic effects. In this study, we found that trypsin treatment decreased the adhesion ability of Lactobacillus plantarum AR326 and AR269, which exhibit good adhesion ability, and surface proteins extracts increased the adhesion of the strains with poor adhesion ability. By SDS–polyacrylamide gel electrophoresis and mass spectrometry analysis, the main component of the surface proteins was detected and identified as a protein of approximately 37 kDa. It was 100% homologous with glyceraldehyde-3-phosphate dehydrogenase (GAPDH) from L. plantarum WCFS1. The adhesion of AR326 and AR269 was decreased significantly by blocking with the anti-GAPDH antibody, and GAPDH restored the adhesion of AR326 and AR269 treated with trypsin. In addition, purified GAPDH significantly increased the adhesion of the strains with poor adhesion ability. These results indicated that GAPDH mediates the adhesion of these highly adhesive lactobacilli to epithelial cells and can be used to improve the adhesion ability of probiotics or other bacteria of interest.
Highlights
Lactobacillus is the most common probiotics which can confer beneficial effects on their hosts when consumed in adequate amounts
After the surface protein extracts were added, the adhesion of AR326 and AR269 treated with trypsin treatment significantly increased
The good adhesion abilities of L. plantarum AR326 and AR269 were significantly decreased after trypsin treatment, and surface proteins extracts can increase the adhesion of AR171 and AR187 with poor adhesion ability and the strains treated with trypsin
Summary
Lactobacillus is the most common probiotics which can confer beneficial effects on their hosts when consumed in adequate amounts These beneficial benefits may include preventing diarrhea (Staudacher, 2015; Sinclair et al, 2016; Kim et al, 2018), modulating the intestinal community (Marteau et al, 2002; Xie et al, 2016), improving lactose tolerance (Mustapha et al, 1997; Staudacher, 2015; Oak and Jha, 2018), reducing the serum cholesterol level (Choi et al, 2015; Kobyliak et al, 2016), suppressing pathogens (Kim et al, 2014; Tuo et al, 2018), and boosting the immune system (Dimitrijevic et al, 2014; Kemgang et al, 2014). The adhesion abilities of lactobacilli have been linked with various different
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