Abstract

The presence of an o -type cytochrome--a pigment previously observed in a number of bacteria, several yeasts, and a parasitic protozoon--was detected from reduced plus CO minus reduced difference spectra of mitochondrial preparations of Tetrahymena . The cytochrome was observed when the reducing agent was ascorbate-TMPD, succinate, DPNH or dithionite and showed absorption maxima at 572, 537, and 418 mμ. It is estimated that about 70–85% of the total cellular o -type cytochrome is located in the mitochondrial fraction, and based on dithionite reduction, its concentration ranges from 0.25–0.62 mμmole per mg. protein in the crude mitochondrial fractions of the several Tetrahymena strains studied.

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