Abstract
By sequential solid-phase immunoadsorption (panning) steps, we have isolated a subset of lymphocytes (comprising 3–7% of rosetted cells) that contains high concentrations of histamine. We have used a radioenzymatic assay for the determination of histamine and have located 117 ng of histamine/1 × 10 6 cells in Leu-5 + (OKT-11), Leu-15 + cells. This subset did not contain basophils and was negative for Leu-4 (OKT-3), Leu-3 (OKT-4), Leu-2 (OKT-8), and 9.3 antigens. The function of this subset of rosetted cells has not been determined.
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