A study to evaluate the correlation between increased homocysteine level and hyperlipidaemia: An observational study

Abstract

: Numerous studies have suggested a possible relation between increased homocysteinemia level and cardiovascular diseases. This relationship is clinically important in the management of cardiac diseases specially in elderly population. However, there is limited data which suggests association between hyperhomocysteinemia and hyperlipidaemia. The aim of this study is to find a relation between hyperlipidaemia and hyperhomocysteinemia in patients who are not on lipid-lowering treatment.A total of 300 hyperlipidemic patients were enrolled who attended medicine OPD. After obtaining informed consent, data were collected by a trained technician according to a standard operating protocol of the laboratory. After an overnight fast of minimum 12 hours, 5 ml of venous blood was taken under aseptic conditions in EDTA tube. The blood was late centrifuged, plasma samples were then separated within half hour of sample collection and were stored at −80 °C in the laboratory. Total cholesterol (TC), Low Density Lipid-Cholesterol (LDL-C), High Density Lipid-Cholesterol (HDL-C) and Triglycerides (TG) were measured on Bene Spheraautoanalyzer. Homocysteine was measured on Robonic ELISA reader.Homocysteine level in blood was directly proportional to increased level of TC, TG, LDL-C and age of the patient. In the same group homocysteine level was inversely proportional to high density lipoproteins. There is strong correlation of increased homocysteine level with hyperlipidaemia.