A study on the relationship between HER-2 gene amplification and protein expression in invasive breast cancer

Abstract

Objective To study the amplification and expression of HER-2 gene and protein in breast cancer and to investigate the relationship between HER-2 gene and Ki-67 ,P53,ER,PR,lymph node metastasis and TNM staging. Methods Fluorescence in situ hybridization （ FISH ） and immunohistochemistry（IHC） method were used to detect the amplification and expression of HER-2 and the expression of Ki-67, P53, ER, PR in 150 cases of breast cancer. Results 49 out of 150 cases were amplificated,positive rate of HER-2 gene was 32. 68%. In the 32 patients whose expression of HER-2 protein was （ ＋ ＋ ＋ ） , HER-2 gene was expressed in 28 cases. In The 46 patients whose expression of HER-2 protein was （ ＋ ＋ ）, HER-2 was expressed in 16 cases. In the 26 patients whose expression of HER-2 protein was （ ＋ ）, HER-2 was expressed in 3 cases. In the 47 cases that the expression of HER-2 protein was negative, there were 45 cases that the expression HER-2 gene was negative. The expression of HER-2 was correlated with ER, PR expression and TNM staging, but not significantly related with age, Ki-67,1）53 and lymph node metastasis. Conclusions FISH and IHC correlate well with each other in the determination of HER-2 gene amplication and protein expression in cases of invasive breast cancer. Key words: Breast neoplasms; Genes, erbB-2; In situ hybridization, fluorescence; Immunohistochemistry