Abstract
A possible significance of carbohydrate moiety in sperm-binding protein of eggs of the sea urchins, Hemicentrotus pulcherrimus and Pseudocentrotus depressus, was examined by the following two experiments. 1. 1. Glycopeptide probably having original carbohydrate moiety was obtained by trypsin-digestion and gel-filtration. It was not functional in sperm-binding at all. 2. 2. Carbohydrate moiety of the original material was oxidized by periodate. Such a protein was fully functional in sperm-binding. It was suggested that the carbohydrate moiety is not essential in the sperm-binding.
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