Abstract
Attempts to remove brush borders from rat intestinal mucosal scrapings in a saline medium are described. Variation of the pH of the medium showed that at about pH 6.5, morphologically intact columnar epithelial cells could be isolated and a suitable method for preparing whole cells is suggested. When the pH was raised to 8.5, the brush borders were liberated. Studies by electron microscopy revealed that a considerable amount of granular cytoplasmic material remained attached to the terminal web. This attached material could be removed, however, by adding EDTA to a suspension of the brush borders.
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Schedule a callMore From: Zeitschrift für Zellforschung und mikroskopische Anatomie
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