A Study on the Effect of Vitamin E on Histomorphological and Immunohistochemical Changes Induced By Electromagnetic Field

Hanafy ,Stefania Magda ,El-Shaer ,E El Attar ,Amany ,Hariri Nahla ,Arafa ,H Manar

doi:10.21608/eajbsz.2019.30681

Abstract

Background: Electromagnetic fields has increased gradually due to technology expansion and thus continues exposure to it may affect the biological system, so that the present work aimed to study the biological effect of non-ionizing radiation magnetic field from power line of 50 Hz which caused by devices become widely used in human activity on the structural properties of cerebral cortex and the possible protective role of vitamin E on it through the histopathological and immunohistochemical study . Result: Thirty mice were divided into three groups, group I (control) , group II animals of this group exposed to extremely low frequency magnetic field (ELF – MF) generated by electrical device (2mT- 20G) for a total period 45 day(2h/day) and group III exposed to the same field and period after adding vitamin E (200 mg /kg) B.W. along with the drinking water. The results showed many changes such as neuronal degeneration and apoptosis in number of Purkinje cells and showing a decreased reactivity in carbohydrates , protein ,DNA and Glial Fibrillary Acidic Protein (GFAP) content in (MF) treated group as compared to control , such changes showed improvement of the histolomorpholigical and immunohistochemical changes in magnetic field (MF) and vitamin E treated group. Conclusion: Electromagnetic fields exposure led to degenerative changes on cerebellar cortex, and the data collected from histopathological and immunohistochemical study revealed a protective effect of vitamin E on cellular cortex layers of the cerebellar cortex

Highlights

Electromagnetic fields (EMFs) expanded in our environment everywhere in our homes, at work, in schools, and elsewhere wherever there were electric wires, electric motors,electronic equipment's, radio-television broadcasting and wireless telecommunications, e.g. cellphones, which considered the major physical contaminants in the environment (Fatma et al, 2011 & Valberg PA et al,2007), this is due to the technological expansion in the last decades
Electromagnetic fields exposure led to degenerative changes on cerebellar cortex, and the data collected from histopathological and immunohistochemical study revealed a protective effect of vitamin E on cellular cortex layers of the cerebellar cortex
Architecture, with normal distribution of different cells : internal granule cell layer with aggregated small deeply stained granule cells, middle Purkinje cell layer showed its large pyriform cells and prominent nuclei in a basophilic cytoplasm which arranged in a single row and surrounded by Bergmann protoplasmic astrocytes and external molecular layer in which Purkinje cell dendrites and granule cell axons interact including neurons, astrocytes and microglial cells (Figure 2, A&B), Group G2 (EMF): Sections showed a demyelination and appearance of areas of degeneration in the molecular layer, showed a variable appearance of degenerated neurons and microglia cells in a few number

Summary

Introduction

Electromagnetic fields (EMFs) expanded in our environment everywhere in our homes, at work, in schools , and elsewhere wherever there were electric wires, electric motors ,electronic equipment's, radio-television broadcasting and wireless telecommunications, e.g. cellphones , which considered the major physical contaminants in the environment (Fatma et al, 2011 & Valberg PA et al ,2007) , this is due to the technological expansion in the last decades. Electromagnetic fields has increased gradually due to Received:3 /2/2019 technology expansion and continues exposure to it may affect the Accepted:5/4/2019 biological system, so that the present work aimed to study the biological effect of non-ionizing radiation magnetic field from power line of 50 Hz. for a total period 45 day(2h/day) and group III exposed to the same field and period after adding vitamin E (200 mg /kg) B.W. along with the drinking water. Acidic Protein (GFAP) content in (MF) treated group as compared to control , such changes showed improvement of the histolomorpholigical and immunohistochemical changes in magnetic field (MF) and vitamin E treated group

Methods

Results

Conclusion