A Study on the Correlation of HLA-DPA1 and-DPB1 Polymorphisms With Nasopharyngeal Carcinoma in the Southern Chinese Han Population

Abstract

Objective To study the correlation of HLA-DPA1 and-DPB1 polymorphisms with nasopharyngeal carcinoma (NPC) in the southern Chinese Han population.Methods From February to July 2012,a total of 74 patients who diagnosed as NPC by pathological examination in Guangxi Wuzhou Red Cross Hospital and Shenzhen Armed Police Hospital were included in this study as study group.And during the same period,a total of 160 healthy donors who were selected by computer randomly in Shenzhen blood center were included in this study as control group (The study protocol was approved by the Ethical Review Board of Investigation in Human Being of our hospital).Both the study group and control group were from southern Chinese Han.All the samples were subjected to sequencing-based typing (PCR-SBT) at exon 2 of HLA-DPA1 and-DPB1 genes in both directions.The allelic frequencies of HLA-DPA1 and-DPB1 loci for the NPC patients were compared with control group by x2 test,the P value and relative risk (RR) value were calculated according to the Wolf method.Results A total of 4 HLA-DPA1 alleles (DPA1 * 01:03,02:01,02:02 and 04:01) were identified in both study group and control group.As for the highly polymorphic HLA-DPB1 locus,14 HLA-DPB1 alleles in the NPC patient group and 18 DPB1 alleles in the healthy controls were identified.The allele frequencies for both HLA-DPA1 and-DPB1 loci showed no significant differences between study group and control group (x2＜3.84,P＞0.05).Conclusions The present study implied that the polymorphisms of HLA-DPA1 and-DPB1 showed no independent association with NPC in the southern Chinese Han individuals. Key words: human leukocyte antigens; HLA-DP; allelic polymorphisms; sequencing based typing; nasopharyngeal carcinoma