A study on phase separation behavior in kappa/iota carrageenan mixtures by micro DSC, rheological measurements and simulating water and cations migration between phases

Abstract

Rheological and thermal properties of mixed kappa carrageenan (KC) and iota-carrageenan (IC) were investigated. KC/IC mixtures showed two-step gelation behavior, with each step coinciding with that observed in one of the two carrageenans. The migration of water and ions between KC and IC solutions separated by a dialysis membrane was also studied. Water migrated from the IC phase to the KC phase following long curing far below the transition temperature of IC and close to the transition temperature of KC. Far below the transition temperature of KC, a much stronger (but slow) depletion of water molecules occurred in the KC phase, concomitant with an increase in the concentration of K+. The results suggest that if phase-separation occurs in mixtures, local polymer and ion concentrations remain similar until the very late stages of gelation. This explains the similar transition temperatures characterizing initial stages of gelation in individual and mixed carrageenan solutions.