Abstract
A study on molecular surveillance of Theileria spp. infection and its impact on hematological and biochemical changes in naturally infected small ruminants at Multan, Pakistan
Highlights
39.5% blood samples were found positive with Theileria infection by microscopic examination and Polymerase chain reaction (PCR) amplification respectively
Malignant ovine theileriosis (MOT) caused due to T. lestoquardi infection resulted in higher mortality in sheep and goats [3], 100% mortalities had been reported in case of malignant ovine theileriosis [5]
The diagnosis of Theileria spp. in general is based on microscopy of stained smears and presence of clinical based symptoms in animals infected with ovine theileriosis
Summary
Theileria infection can be acute, subacute or chronic. Malignant ovine theileriosis (MOT) caused due to T. lestoquardi infection resulted in higher mortality in sheep and goats [3], 100% mortalities had been reported in case of malignant ovine theileriosis [5]. The diagnosis of Theileria spp. in general is based on microscopy of stained smears and presence of clinical based symptoms in animals infected with ovine theileriosis. The significant decrease in RBCs counts, Hb contents and PCV values were identified while no changes found in MCH and MCHC values in infected goats with theileriosis [14]. 5 ml collected blood was poured in eppendorf having few drops of EDTA for extraction of DNA for PCR amplification; while other 5 ml collected blood was used for serum collection in order to determine hematobiochemical analysis. Haematological and Serum biochemical studies The haematological values such as RBCs, WBCs, HB concentration, PCV, MCV, MCH and MCHC values were recorded of infected and healthy small ruminants. One way Analysis of Variance (ANOVA) was used to compare and determine significant difference of hematological and biochemical parameters of laboratory obtained values between healthy and infected animals with theileriosis.
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