A study on in vitro antioxidant activity of aqueous seed extract of sesbania sesban (l) merr.

Kathiravan S Kathiravan S,Shwetha V Kalava Shwetha V Kalava

doi:10.26524/krj.2021.21

Abstract

The present study was done to investigate the in vitro antioxidant activity of aqueous extract of Sesbania sesban seeds. The assays such as DPPH, Chelation, ferrous ion, ABTS, Superoxide radical, hydroxyl radical assay, FRAP assay and total antioxidant activity were done to assess the antioxidant potential of the seed extract. The extract was tested at a concentration range of 100 – 500 μg/ml for all the assays and the values were compared with a standard. The results obtained showed that the radical scavenging activity was in a dose dependent manner and found to increase with increase in concentration of the extract. The IC50 value was calculated for the assays and tabulated for inference. Different assays revealed different levels of radical scavenging potential of the extract and exhibited as a better antioxidant source for therapeutic applications.

Highlights

The traditional medicine all over the world is nowaday revealed by an extensive activity of researches on different plant species and their therapeutic principles
In the DPPH test, the stable, nitrogen centered, coloured, DPPH free radical is reduced either by hydrogen donor or antioxidant to a nonradical DPPH-H and the decrease in colour of DPPH radical is monitored over a time period
The seed extract exhibited stronger radical scavenging ability and its 50% inhibition reached at 265 ± 12.5 μg/ml which indicates its good antioxidant potential

Summary

Introduction

The traditional medicine all over the world is nowaday revealed by an extensive activity of researches on different plant species and their therapeutic principles. Free radicals and reactive oxygen species (ROS) such as superoxide, hydroxyl and peroxyl radicals are normal by-products of aerobic metabolism produced in vivo during oxidation. These ROS are generated in the mitochondria and microsome organelles under normal physiological conditions. Overproduction of ROS can result in oxidative damage to various biomolecules including lipids, proteins, DNA and cell membranes. They lead to the development of a variety of diseases such as coronary heart diseases, cancer, diabetes, hypertension and neurodegeneration. The activities of these endogenous defense systems may not be sufficient to mop up the free radicals [2]

Methods

Results

Conclusion