A Study on IFN-γ and IL-10 Gene Expression Changes in Gallus gallus domesticus Embryo Infected with E. coli and its Possible Alteration by Bakreshwar Hot Spring Water

Abstract

In search of effective treatment against E. coli there are some conventional medicinal treatments available but it becomes difficult to fight against MDR strains as they are resistant to most of the antibiotics. It was found that Bakreshwar hot spring water has some therapeutic properties against various infections, mostly against skin infections. Bakreshwar hot spring having a pH range of 8.1-8.5 and temperature which varies from 35°-71°C, contains several chemical compounds. Our aim of this work was to study whether Bakreshwar hot spring water can cure infections caused by E. coli in Gallus gallus embryo in relation to their gross appearances and IFN- γ and IL-10 gene expression changes. The sample water was collected around March, 2023. To study the therapeutic activity of Bakreshwar hot spring water, different experimental sets were set up with 14days embryonated eggs. Freshly prepared 0.5Mcfarland E. coli culture was inoculated into the eggs and in curative set, 100µl hot spring water was injected to check any alterations. Allantoic fluid was collected and mRNA was extracted the very next day. cDNA was synthesized from the extracted mRNA. Along with master mixture (primers, nuclease free water, Syber green) the cDNA was run at RT-PCR and CT value was obtained. The mean gene expression shows that IL-10 and IFN- γ both gene expression was decreased in curative set after treatment with water as compared to control sets. These finding suggests that Bakreshwar hotspring water treatment can be a potential method to control E. coli caused infections.