Abstract
During a study period, total 9 localities i.e.Darlaghat (1563m), Jatoli (1464m), KotlaPanjola (1190m), Chandol (1418m), Dhar (1360 m), Alsindi (1132 m), Naldehra (2050 m), Ghanahatti (1668 metres) and Potters hill (1887 m)of Punicagranatum were selected for the field surveysfrom which Eristalis tenax was observed on the Punicagranatumatfour localitiesi.e. Darlaghat (1563 m), Naldehra (1887 m), KotlaPanjola (1190 m) and Pottershill (1418 m) and samples of species were collected.All the sampled species of Eristalis tenax four localities were characterized genetically through mtCOI gene for the intraspecific diversity studies. Multiplesequence alignment between all the mtCOI sequences of Eristalis tenaxrevealed the similar pattern between the nucleotides of all the sequences.Nucleotide content analysis of all the species at first, second and third codon position revealed theaverage A+T percentage (69.14%) was higher than C+T (30.86%). The A+T bias was prominent for all codon sites. Itwas found that transition/transversion value of COI (R) is 0.50 which concluded that there is insignificant neutral selection in samples of Eristalis tenax of Himachal Pradesh. Phylogenetic analysis studies showed that the sample of Naldehra and Darlaghat were phylogenetically similar to each other and the sample of Pottershilland Darlaghat were found to be quite dissimilar from each other.
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