Abstract
Ultrastructural and limited ultracytochemical studies were explored with potassium ferrocyanide-reduced osmium tetroxide (POCC). The use of 2% osmium tetroxide solution following POCC treatment was found to improve the preparation of ultrathin sections by facilitating the infiltration of Epon into the treated tissues. Potassium ferrocyanide alone did not stain tissues. Treating of tissues with potassium ferrocyanide and osmium tetroxide separately was not effective indicating that reduced osmium staining could not be produced within the tissues.
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