Abstract
Dissociation of neutralizing antibody from Coxsackie B4 virus was achieved by acid treatment; pH 2.5 was found to be optimal for the reaction. Reactivated virus could be reneutralized by the dissociated antibody, but at a slower rate than the initial neutralization. Simple dilution was an effective method for recovering infectious virus from virus-antibody mixtures only if these mixtures were first adjusted to an acid pH. In the presence of excess antibody, recovery of infectious virus increased five- to sevenfold if the acidified virus-serum mixture was diluted in hypertonic rather than isotonic saline.
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