A Study on correlation between the drug resistance and biofilm production among the GNB isolated from blood

Abstract

Introduction: Now days many drug resistant bacteria has been isolated from bacteraemia cases. And these bacteria threaten the patient’s life with associated biofilm production around the implants like Intravenous catheters, Venflons, etc. Aim: to identify the prevalence of drug resistance among GNB (Gram Negative Bacteria) isolated from blood culture and to check their ability to produce biofilm by invitro method. Materials and Methods: A total of 365 blood samples were collected and processed from clinically diagnosed septicaemia patients. These samples were processed to isolate the bacteria with ABST by using standard CLSI guidelines. Biofilm production was identified by Tube method, Congo red agar method and Tissue culture plate method. Result: Out of 365, 62 (17%) blood samples were isolated with gram negative bacilli. Out of 62 isolates, 17 (27.4%) were identified as Escherichia coli, 13 (21%) were identified to be Acinetobacter spp, 12 (19.3%) were Pseudomonas spp, 08 (13%) were Salmonella spp, 06 (9.6%) were isolated as Klebsiella spp. Citobacter spp and Enterobacter spp were isolated with 03 (4.8%) each. Among these 62 isolates, 28 (45.2%) were identified to be either ESBL or MDR isolates, 18 (29%) isolates produces biofilm by all three methods. Among the biofilm producers, (55.55%) were found to be the drug resistance bacteria. Conclusion: Result of this Blood culture reveals the increased rates of drug resistance bacteria which were due to improper use of antibiotics. And the major drug resistant bacteria are associated to produce Biofilm which makes the treatment to be more challenging. Key words: Biofilm production, Septicaemia, ESBL & MDR isolates, Drug resistance