A study on bacteriological profile and antimicrobial resistance pattern from various body fluids of patients attending the tertiary care Hospital, KIMS, Hubli

Abstract

Introduction: Body fluids like pleural fluid, ascitic fluid, cerebrospinal fluid etc are usually sterile, but they can get infected by different micro organisms, thus leading to life threatening infections.1,2 Aims and Objectives: 1) To isolate the causative organisms of sterile body fluid infections. 2) To determine the antimicrobial resistance pattern of the isolates. Materials and Methods: 1) This is a retrospective observational study, conducted from January 2017 to December 2017 in the department of Microbiology KIMS Hubli. 2) Sterile body fluids were processed according to Standard laboratory Procedures. 3) Antimicrobial susceptibility testing was performed by Kirby-Bauer disc diffusion method as per CLSI guidelines. Results: A total of 635 samples were studied out of which 233(36.6%) were pleural fluids, 222(34.9%) were ascitic fluids, 174(27.4%) were cerebrospinal fluids and 06(0.9%) were pericardial fluid. In our study the predominant organisms were E-coli (23.23%) and NFGNB (19.01%) followed by Pseudomonas (14.08%), Klebsiellaspp (13.38%), S.aureus (10.56%) and Citrobacter spp (7.04%). Gram negative isolates were 100% sensitive to Imipenem followed by Amikacin (78%), Gentamicin (74%), Cefepime (69%). The Gram positive isolates were 100% sensitive to Vancomycin and Linezolid followed by Gentamicin (96%), Cefepime (94%), Amikacin (92%). The Pseudomonas isolates were highly sensitive to Imipenem (96%) and Piperacillin Tazobactum (92%). High resistance was observed to Ceftazidime, Cefoperazone and Levofloxacin. Conclusion: Knowledge of bacteriological and antimicrobial profile of sterile body fluids is necessary, so that the life threatening infections can be effectively treated and thus to prevent antimicrobial resistance associated with it. Keywords: Sterile Body fluids, Antimicrobial resistance, E-coli, NFGNB, Kirby-Bauer disk diffusion method.