A STUDY ON BACTERIOLOGICAL IDENTIFICATION OF SUB-GINGIVAL PLAQUES IN PERIODONTITIS PATIENTS

Abstract

Objective: The current study's objectives were to isolate and characterize the periodontal pathogens from subgingival plaque obtained from patients with chronic periodontitis using conventional microbiological techniques.
 Materials: In the Department of Microbiology at a Tertiary Care Teaching Hospital was where this study was carried out. Over the course of six months, from January to June 2019, samples were taken from subgingival pockets in patients with chronic periodontitis who visited the periodontology outpatient department at our institute of dental sciences. There were 21 cases in the research. For the purpose of preventing saliva contamination, tooth surfaces were dried using sterile gauze. Subgingival plaque samples were taken from the majority of diseased sites using a sterile periodontal Gracy curette, put in a test tube with fluid thioglycollate medium, and taken to the microbiology lab where they were processed right away using conventional microbiological procedures.
 Results: E. coli grew in the majority of samples (9), followed by Pseudomonas species and Staphylococci in 7 samples. Only sample number 7 out of 21 showed no growth for Candida albicans; the others all exhibited development. Samples like Sample 1, 5, 6, 8, 10, 11, 12, 13, 15, 16, 17, 18, 19, and 20 produced isolates that were multidrug resistant.
 Conclusion: Therefore, while developing a treatment plan for adult patients with periodontitis, the microbial diversity discovered in the current study should be taken into account. Variations in the quantities and species of cultivable bacteria have been found in investigations of periodontitis patients from different geographic regions, including developed and developing countries.