Abstract
The tissue culture technique was selected for Cinnamomum longepaniculatum to breed excellent nursery stock, by analyzing and comparing the influence of disinfectant varieties, concentration and action time, MS medium, 6-BA, NAA and IBA on tissue culture of annual shoots. The results showed that contamination rates were no higher than 15% and survival rates were higher than 70%, under 10% of 84 disinfectant treatment for 15min, or 15% of 84 disinfectant treatment for 10 min. The optimum medium for adventitious bud regeneration was sucrose 30 g·L-1+carrageenan 6 g·L-1+MS+6-BA 1.0 mg·L-1+ IBA 0.1 mg·L-1; The medium for multiplication of adventitious buds was sucrose 30 g·L-1+carrageenan 6 g·L-1+MS+6-BA 1.0 mg·L-1+NAA 0.10 mg·L-1+IBA 0.2 mg·L-1; The rooting medium was sucrose 15 g·L-1+carrageenan 6 g·L-1+1/2MS+IBA 1.5 mg·L-1+NAA 1.0 mg·L-1.
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