Abstract
Chicken embryo lethal orphan (CELO) virus DNA (molecular weight 28.3 × 10 6) was cleaved by the restriction enzyme EcoR1 into seven fragments whose molecular weights ranged from 1.8 × 10 6 to 10.2 × 10 6 as measured by gel electrophoresis and electron microscopy. 32P-labeled CELO virus DNA was treated with EcoR1 and the resulting fragments were separated by gel electrophoresis. The kinetics of renaturation of the isolated fragments was measured in the presence of DNA extracted from a line of CELO virus-transformed hamster skin (THS) cells and from control hamster cells. THS cell DNA contained sequences homologous to only six of the seven EcoR1-produced CELO virus DNA fragments. No sequence homologous to a 2.0 × 10 6 molecular weight fragment was detected indicating that the DNA of THS cells did not contain a complete copy of the virus genome.
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