Abstract
Fat metabolism is closely related to the economic characteristics of beef cattle. Therefore, regulating fat deposition and increasing intramuscular fat deposition are among the main goals of breeders. In this study, we aim to explore the regulatory role of CB1 gene on PPARγ2/PLIN1/HSL pathway in fat metabolism, and to further explore the differential expression of regulatory factors of this pathway in Shandong black cattle and Luxi cattle. In this study, CB1 overexpression stimulated lipid synthesis in adipocytes to some extent by increasing the levels of FASN and ACSL1. CB1 inhibitors reduce the lipid content in adipocytes and reduce the expression of GLUT1 and Insig1. In addition, overexpression of CB1 decreased the expression of PPARγ2 and led to an increase in PLIN1 expression and a decrease in HSL expression in adipocytes. We also found that the CB1/PPARγ2/PLIN1/HSL was differentially expressed in the different breeds of cattle and was involved in the regulation of fat metabolism, which affected the fatty acid content in the longissimus dorsi muscle of the two breeds. In short, CB1 participates in lipid metabolism by regulating HSL in the PPARγ2 and PLIN1 pathways, and improves lipid formation in adipocytes. In conclusion, CB1/PPARγ2/PLIN1/HSL pathway may be involved in the regulation of lipid metabolism.
Highlights
Fat metabolism is closely related to the economic characteristics of beef cattle
The results from the analysis showed that FABP4, PCK1, ADIPOQ, hormonesensitive lipase (HSL), SCD5, PIK3R3 and perilipin 1 (PLIN1) were enriched in five pathways
The construction of the regulatory network of fat metabolism was based mainly on two types of data: the data from the transcriptome database and the research results established in our laboratory and the data obtained from information related to fat metabolism in literature databases such as NCBI, CNKI and Ensembl
Summary
Fat metabolism is closely related to the economic characteristics of beef cattle. The deposition of animal body fat represents a balanced state of fat synthesis and catabolism. Once the original balance is destroyed, fat deposition is increased or body fat is reduced, affecting the meat quality of animals. Regulating fat deposition and increasing intramuscular fat deposition are among the main goals of breeders. Increasing research is focused on the mechanism. CB1/PPARγ2/PLIN1/HSL Pathway of animal fat deposition and its regulation to influence the processes of animal fat deposition, improve carcass quality and increase production levels
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