Abstract
Young rats were maintained for 84 days on a diet which supplied to each 0.15 mg of iron and 4.26 mg of calcium per day. The Ca:P ratio of the diet was 1.4. For some of the animals the dihydrate of disodium ethylenediaminetetraacetic acid (EDTA) was added at a level of 0.01%, which supplied 1 mg per rat per day. The molar ratio of EDTA:Ca:Fe was 1:37.9:1.The EDTA did not affect the growth of the rats. It did not affect the excretion of calcium, or its concentration in blood serum or femur. Since the faecal excretion was not affected, apparently the absorption was not impaired. The growth of the femur was not affected. EDTA was associated with generally higher levels of iron in the serum, but the average difference was not statistically significant. There was no effect upon the liver iron or the haemoglobin level in the blood.Small amounts of EDTA in the diet apparently did not interfere with the utilization of low and probably inadequate dietary levels of calcium and iron.
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