Abstract
A selection of food grade soybean varieties from Canada and Japan, and an experimental line which produces a high proportion of ‘stone seeds’, were separated into seed coat and cotyledon. Following the removal of oil and protein, the samples were fractionated into polysaccharide groups, first by solubility in water and then secondly in a scheme using aqueous ethanol, sodium carbonate and KOH. The subfractions were hydrolysed using 1 M and 12 M sulfuric acid then analysed by HPLC for monosaccharides and by a colourimetric method for uronic acids. The results showed that there was very little intervarietal difference in the carbohydrate composition of the cotyledons but there were some significant differences in the seed coat composition. A high concentration of xylose was found in the KOH soluble hemicellulose fractions which was highest in the stone seed sample and lowest in the Japanese varieties.
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