A study of the initial adhesive force of cells on silk fibroin-based materials using micropipette aspiration.

Xiaojie Lian,Yan Wei,Rui Xu,Shichao Liu,Hesun Zhu,Di Huang,Song Wang,Liming Liu,Yali Wu,Quanyou Zhang,Miaomiao Du,Qiang Lu

doi:10.1093/rb/rby004

Xiaojie Lian, Yan Wei + Show 10 more

Open Access

https://doi.org/10.1093/rb/rby004

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Abstract

With the development of biomaterials, more attention is paid to the adhesion characteristics between cells and materials. It is necessary to study the adhesive force with a suitable method. Silk fibroin (SF) is widely investigated in biomedical application due to its novel biocompatibility and mechanical properties. In this article, the micropipette aspiration method and measurement pattern of uniform cells in round shape (UCR) was used to study the initial adhesive force of three types of cells on pure silk fibroin films (SFFs). We also compared the adhesive forces of modified SFFs with that of pure SFFs. The results of adhesive force in the initial adhesive stage were in concordance with the results of MTT assay and microscope observation, which were confirmed by the above three cell lines and four kinds of SFFs. The results indicated UCR was an efficient and quantitative measurement pattern in initial adhesion stage. This article also provides a useful method in identifying initial cell-materials interactions.

Highlights

Cytocompatibility is one of the most important aspects of biocompatibility of biomaterials [1,2,3]
The culture time might be different depending on different cell types and cell batches, which should make sure a few round shape cells adhering on surfaces in all groups
The results indicated that there might exist initial adhesion stage in force, when the initial adhesive forces of NIH-3T3, Osteoblasts and ECV304 was (16.1 6 8.5), (4 6 3.4) and (7.1 6 0.7) nN on silk fibroin films (SFFs) respectively, there was a significant difference between the cell adhesive forces of tissue culture polystyrene dishes (TCPS) and SFF

Summary

Introduction

Cytocompatibility is one of the most important aspects of biocompatibility of biomaterials [1,2,3]. Micropipette aspiration technique was utilized to investigate the adhesive force of a single cell quantitatively. The measurement pattern of uniform cells in round shape (UCR) was already used in evaluating the endothelial cell adhesive properties on various silk fibroin (SF)based materials in our previous study [4, 5]. This method shows high reproducibility and sensibility in evaluating the cellular affinity quantitatively with a small amount of uniform cells in earlier adhesion stage. The purpose of this study is first to investigate the adhesive force of fibroblasts (NIH-3T3), osteoblasts and endothelial cells (ECV304) on pure silk fibroin films (SFFs) and tissue culture polystyrene dishes (TCPS) using the UCR measurement pattern

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