Abstract
Background.Soybean (Glycine max (L.) Merr.) gradually becomes one of the leading legume crops in Kazakhstan. The area under soybeans in the country has been increasing annually and requires the development of adapted cultivars with a higher yield, improved quality characters, and resistance to emerging fungal diseases. The enlargement of the crop’s gene pool also suggests the need to study and document local soybean accessions to meet the standards of the available world soybean collection by using reliable and informative types of DNA markers.Materials and methods. In this study, the soybean collection consisting of 288 accessions from different countries, including 36 cultivars and promising lines from Kazakhstan, was studied. The molecular genetic analysis was performed using nine polymorphic SSR (simple sequence repeats) markers, seven of which (Satt244, Satt565, Satt038, Satt309, Satt371, Satt570 and Sat_308) were associated with resistance to three main fungal diseases of soybean – frogeye leaf spot, fusarium root rot, and purple seed stain.Results. The average PIC (polymorphism information content) value of the analyzed SSR markers constituted 0.66 ± 0.07, confirming their highlevel polymorphism. The principal coordinate analysis suggested that the local accessions were genetically most close to the accessions from East Asia. As the collection showed a robust resistance to three studied fungal diseases in Almaty Region during 2018–2019, the distribution of the studied SSR markers in the population was not significantly associated with resistance to the analyzed diseases under field conditions.Conclusion. SSR genotyping of the soybean collection helped to identify accessions that potentially possess resistance-associated alleles of fungal disease resistance genes. The data obtained can be further used for the development of DNA documentation and the breeding the promising cultivars and lines of soybean.
Highlights
Soybean (Glycine max (L.) Merrill) is one of the most economically important legume crops, which provides plant protein for more than a quarter of all food and feed in the world (Garcia et al, 1997)
The data were used to study the relationships between the disease resistance of the collection accessions and the following 8 major agronomic traits: plant height (PH), the height of lowest pod (HLP), number of lateral branches (NLB), number of fertile nodes (NFN), number of seeds per plant (NSP), thousand seeds weight (TSW), yield per plant (YP), and yield per plot (YpP)
Field trials of the soybean collection consisting of 288 accessions from different parts of the world suggested that over 97% of the collection was resistant to FLS, FRR and PSS fungal diseases in the studied period
Summary
Soybean (Glycine max (L.) Merrill) is one of the most economically important legume crops, which provides plant protein for more than a quarter of all food and feed in the world (Garcia et al, 1997). For further development of the soybean industry, the Government of Kazakhstan has declared a new program “Northern Soybean” for diversification of soybean to the north part of the country. Implementation of this program would make it possible over five years to enlarge sown areas to 1.5 million ha, raise productivity, and increase the production over 3 million tons (North Kazakhstan..., 2019). The area under soybeans in the country has been increasing annually and requires the development of adapted cultivars with a higher yield, improved quality characters, and resistance to emerging fungal diseases. The soybean collection consisting of 288 accessions from different countries, including 36 cultivars and promising lines from Kazakhstan, was studied. The data obtained can be further used for the development of DNA documentation and the breeding the promising cultivars and lines of soybean
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
