Abstract
PURIFICATION of total neutral fractions of urinary steroids is now generally recognized as necessary to overcome the atypical colors caused by urinary pigments and chromogens in the Zimmerman and Pincus reactions. This is not so important in the latter procedure since the maximum absorption of blue compounds formed by the antimony trichloride reagent is distinctly different from that of contaminating pigments and yellow chromogens. The Girard reagent is generally used for the separation of the non-“ketonic from the ketonic fraction and provides a more accurate estimate of the 17–ketosteroid content. It nevertheless has certain disadvantages in that a loss of 17–ketosteroids occurs, and the ketonic fractions obtained are seldom free from pigment and nonketonic chromogens and frequently give the same atypical colors observed n i unfractionated extracts. A better method seems to be that of adsorption on magnesium oxide and elution with ether (Bauman and Metzger, 1940).
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