A study of the effect of PTFE electret on fibroblast cell cycle with flow cytometry (FCM)

Abstract

In this paper, the PTFE films were respectively charged at /spl plusmn/300 V, /spl plusmn/500 V and /spl plusmn/1000 V by corona charge system. After applied on the cells for 24 h, 48 h and 72 h, the influence of electret on fibroblast (3T3 cell) cell cycle was studied with flow cytometry. The results indicated that 1) after action of negative PTFE electret field for 24 h, the cell number in G/sub 1/ phrase and G/sub 2/ phrase decreased, but increased in S phrase. It suggested that negative electret can improve cell's growth. 2) negative electret can not only enhance the growth of cells, but also enhance the apoptosis of mutant cell. 3) after action of positive electret field on fibroblast for 24 h, the cell number in G/sub 1/ phrase increased, while the cell number decreased in S and G/sub 2/ phrase. Positive electret can block some cell in G/sub 1/ phrase. So it can restrain the growth of fibroblast cell. In short, electret has bilateral regulation effect on the enhancement and block of cell growth.