Abstract

Measurement of the cytochrome content of cells grown under different aeration conditions showed that cytochrome o did not go through the same adaptive changes as did cytochromes a 1 and a 2, and no correlation was found between cytochrome content of cells and their potential respiration rate. Cells grown at low growth rate demonstrated increased content of cytochrome a 2. A peak of absorption at 500 nm was observed in cells grown at 0.04 h -1 and in samples incompletely oxidised. Kinetic studies using a dual-wavelength spectrophotometer showed two types of absorption at 500 nm. One type appeared on aerating anaerobic cells and disappeared slowly with time, and the other appeared transiently as the cells became anaerobic.

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