Abstract

Natural intercourse (NI) or artificial insemination (AI) was performed in 17 cooperative couples, whose infertility might be due to inadequate patency of tube in the periovulatory or ovulatory phases, before tubal plastic surgery was carried out. Successive examinations were made for the presence and motility of sperm in the oviduct during the surgery.(1) Among the AI group if insemination was performed 2 hours-4 days before the operation, sperms were present in the oviduct.Among the NI group if the couple had intercourse 2 to 4 days before the operation, sperms were present in the oviduct.(2) There was no significant difference between the patients in whom in-adequate patency of tube was found and the control group, in the groups excluding hydrosalpinx (HYD) group, as far as the presence of sperm in oviduct is concerned.In the HYD group, the rate of the presence of sperm was low and in the group in which chronic inflammation of the oviductal lumen was observed histologically, few motile sperms were seen in vivo.(3) Successive observation of normal sperm in vitro in HYD fluid, serum, Tyrode's solution, seminal plasma, and physiological saline showed marked decreease in the sperm motility rate in HYD fluid and physiological saline.(4) As an important factor of these phenomena, it is recommended that lowering of glucose concentration and albumin concentration in HYD fluid is considered.

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