A STUDY OF PATIENTS WITH GALL BLADDER CARCINOMA FROM THE ANGLE OF PLOIDY PATTERN OF NUCLEAR DNA

Abstract

In our treatment of 51 patients with gallbladder carcinoma, macroscopic curative resection could be performed on 28 patients. The volume of nuclear DNA was measured in these 28 patients with the use of cytometer, and a study was made of the relationship of the ploidy pattern of the nuclear DNA with clinicopathologic aspects and late results. Of the 28 patients, 14 had D type (diploid) of ploidy pattern, while the other 14 showed N (non-diploid) pattern. N type was more commonly found in poorly differentiated carcinomas than D type. Invasion into the vein and lymphatic vessel positive cases were more frequentry noted in N types. There was a significantly larger number of cases with invasion into the perineural space in N type group (p<0.05). All early carcinomas were of N type, but all advanced carcinomas of D type. No lymph node metastasis was found in D type carcinomas, but it was noted in 50% of N type carcinomas, with a significant difference (p<0.05). D type group had significantly better late result than N type group (p<0.001). In the invasion depth of ss, late result of D type carcinomas was favorable than that of N types. It is concluded that the ploidy pattern of the nuclear DNA can be a valuable index for assessing the biological malignancy as well as a determinant factor for prognosis.