Abstract
A very simple experimental setup for a Dynamic Light Scattering measurement was used to measure the average size of the milk proteins in aqueous suspensions at 20 °C. The PH of the suspensions was adjusted using Calcium lactate, in its most common form of pentahydrate C6H10CaO6•5H2O. The mean size variation of the suspended particles in time has been monitored and reveals a fast increase over a time interval of less than ten of seconds.
Highlights
Bovine milk, contains fat globules with a diameter of about 2 μm, casein micelles of about 0.2 μm, whey proteins of 0.006 μm, and many other small molecules having the diameter less than 0.002 μm [1]
Following the preparation steps described in subsection 2.1, the samples were placed in the circular glass tube after dilution
We notice that the small variation of the diameter with the change of the pH is quite fast, within the first 9 seconds after the pH change was produced, and we notice this feature from the plot in Fig. 3, where the diameters at t=9s are already shifted from the initial values, at t=0, which were around 110 nm, considering the errors in determining them using Dynamic Light Scattering (DLS) time series processing
Summary
Bovine milk (milk hereafter), contains fat globules with a diameter of about 2 μm, casein micelles of about 0.2 μm, whey proteins of 0.006 μm, and many other small molecules having the diameter less than 0.002 μm [1]. About 80% of bovine milk protein is called casein, while the rest are named whey protein [2]. The medical drug industry and traditional industrial food industry use the self-assembly processes under different physical conditions, as temperature and pressure and chemical conditions as pH. For this reason, studying the dynamics of the milk protein size influence of the pH might be of interest. As the aggregation or denaturation can undergo quite fast, it is of interest to use an optical method, especially one that can be used in situ, without removing the sample from the protein aggregation reaction vessel and without using reagents that can modify the pH of the solution
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