A study of microbial pattern and antibiotic susceptibility in blood cultures among children aged upto thirty six months

Abstract

Background: Sepsis is the most common cause of high morbidity and mortality among newborn, infants and young children. The organisms implicated in these infections vary with geographical alteration.so antibiotics used should be decided by local prevalence of microbial pathogen, and its bacterial susceptibility pattern. Methods: a prospective observational study among the different neonates and children aged 0 to 36 months. Blood samples are taken under aseptic precaution. Inocluted onto blood culture media (brain heart infusion broth). It is incubated at 37°C. Turbidity is observed daily and it is sub cultured on alternate days. If there is growth, the organism is identified by routine biochemical reactions and the antibiotic susceptibility test was done on the Muller Hinton agar using appropriate antibiotic discs. For testing antibiotic susceptibility, criteria defined by the clinical and laboratory standards institute (CLSI) were followed. Results: Among 148 subjects 102 found to be culture positive with positive rate of 69%. Gram negative organism is more prevalence in this study, most common isolates from gram negative sepsis was Klebsilla species (22.2%) followed by E. coli (18.1%). Conclusions: Gram negative organism form the majority of isolates in our setup with Klebsilla as the most common species among them, which was least sensitive to most of the drugs. Among gram positive organism MRSA as the most common isolates in our setup. Most of the antibiotic were sensitive. Limited and objective use of antibiotic therapy is a much-needed statergy and the new guidelines.