A study of ion-neutral collision cross-section values for low charge states of peptides, proteins, and peptide/protein complexes

Abstract

Here, we report ion-helium collision cross-sections (CCS) for a number of peptide, small protein, and peptide/protein ionic complexes. The CCS values reported here are compared to previously reported results [1,2]. We also compare values for low charge state species, i.e., [M+H] + and [M+2H] 2+, formed by MALDI with values for high charge state species formed by ESI, and the measured CCSs are compared with predicted CCS for solid-state and solution phase structures and calculated structures obtained by using a protein–protein structure algorithm generator, based on a combined Biomolecular complex Generation with Global Evaluation and Ranking [3] and Multi Dimensional Scaling [4].