A study of gluconeogenesis by the administration of 2-deoxyglucose. I

Abstract

2-Deoxyglucose (2DG) an inhibitor of glucose metabolism, 1 induced hyperglycemia and increased endogenic gluconeogenesis. These effects are seen 5–10 minutes after the administration of 2DG. Two hours later an increased liver glycogen content was found. A rise in ninhydrin-reactive-substances was observed in serum and muscle during the first 20 minutes after 2DG administration. This was followed by a decline. A constant rise in urea excretion was detected beginning 30 minutes after the administration of 2DG. The effect of 2DG was not seen in anesthetized rats. On the other hand, electrical stimulation of the posterior hypothalamus increased gluconeogenesis. 24 The inhibitory effect of sympathetic blocking of this process depends on the nutritional state of the animal. The adrenals were indispensable to the increase of gluconeogenesis. A rise in gluconeogenesis was demonstrated 15 minutes after glucagon administration. The mechanism of acute gluconeogenesis following administration of 2DG is discussed.